Abstract:
Genetic polymorphism might be consider as a marker for predisposition of different diseases
including leukemia. Glutathione S Transferase has role in metabolism of different types of
Xenobiotics to facilitate their excretion from the body. It has also role in the metabolism of
the drugs used in chemotherapy for different diseases. There are many genes that exhibit
polymorphism but most of them show single nucleotide polymorphism. The deletion of
whole gene is rare. GSTM1 and GSTT1 members of glutathione family are polymorphically
deleted. Purpose of the study was to investigate deletion of these two isozymes in local
Pakistani population and to investigate if any relationship exists in leukemic population. Male
and females of healthy and leukemic patient volunteers were selected. Leukemic population
was further divided into different groups (ALL, CLL, AML and CML).
Biochemical parameters and oxidantion status of all patients and healthy volunteers were
measured by kit method and Genetic polymorphism was seen by PCR and gel
electrophoresis.
Total bilirubin, creatinin, urea and ALT is greater in patients compared to healthy volunteers.
Total oxidant status was low while total antioxidant status was high in healthy volunteers
compared to patients depending on disease condition.
Healthy subjects show 54% deletion of genes (66% and 52% in female and male
respectively). Patients show 67% deletion of GSTM1 gene in which ALL 66% (68% and
76%, CLL 70% (80% and 60%), AML 70% (72% and 68%) and CML 62% (60% and 64%)
in female and male respectively.
GSTT1 deletion found healthy volunteers was 71% and it is 72% and 70% in female and
male respectively and was found 70% in leukemic patients. ALL 78% (76% and 80%), CLL
76% (80% and 76%), AML 66% (76% and 76%) and CML 60% (56% and 64%) female and
male respectively. All patients and healthy volunteers have more than 50% deletion of
GSTM1 and GSTT1 genes. More deletion of GSTM1 was seen in patients than healthy
volunteers while local population seems to be more prone to leukemia and may respond less
to some chemotherapeutic drugs.
