Hyperactivation and Thermostability Enhancement of LiP from Ganoderma Lucidum IBL-05 through different Immobilization techniques for Industrial Application

Abstract

Under appropriate growth conditions, white rot fungi like Ganoderma lucidum produce Lignin Peroxidase (LiP) as one of the extracellular oxidative enzyme that can oxidize a wide range of naturally stubborn musky pollutants. However, some important drawbacks are experienced in employing free enzymes, including unstable activity under working conditions and separation of the enzyme from reaction product. Technology in which enzyme attached to solid support has proofed an operative solution to these issues. Immobilization improves enzyme catalytic assets and functioning stability and assists enzymes various salvage, parting, and continuously automatic operations in industrial production. In this project lignocellulosic agro-industrial waste material, wheat straw was used as carbon source and solid support for LiP production by an native WRF strain Ganoderma lucidum IBL-05 through solid state fermentation under pre-optimized growth conditions. to 3.1 to 7.9fold and 2213.78 U/mg specific activity. The presence of single band (38 kDa) on sodium dodesyl sulfate gel electrophoresis define that the LiP was monomeric single aminoacid chain. Partialy purified LiP was immobilized by adopting different immobilization techniques like Ca-alginate beads, chitosan beads, silica gel, activated animal charcoal, alginate-chitosan microencapsules and CLEAs. The influence of temperature, pH, and substrate concentration on free and immobilized LiP was investigated to determine the effect of immobilization on LiP activity and stability. Silica (93%) was the best support in which LiP gave maximum immobilization efficiency. Energy-Dispersive X-ray spectroscopy (EDX) was done for conformation of immobilized LiP in the support. Results of EDX showed that LiP was successfully immobilized in different support material. The immobilized LiP was used for degradation of textile dyes and textile effluents. Maximum textile dye removal was done by activated animal charcoal (95%) and maximum textile industry effluents decolorization was done by silica (98%). Immobilized LiP was more effectual decolorizer of selected textile dyes and practical textile industry effluents.