dc.contributor.author |
AFSHAN, KIRAN |
|
dc.date.accessioned |
2017-12-06T05:28:38Z |
|
dc.date.accessioned |
2020-04-15T04:37:33Z |
|
dc.date.available |
2020-04-15T04:37:33Z |
|
dc.date.issued |
2014 |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/11847 |
|
dc.description.abstract |
Fasciolosis also known as fascioliasis is a highly pathogenic liver infection
caused by F. hepatica and F. gigantica. The study was aimed to use morphological
markers for identification of fasciolids, generation of risk maps and use of rapid
and cost effective diagnostic tool to reduce the impact of fasciolosis on animal
health. The phenotypic features of adults and eggs of fasciolid species infecting
buffaloes from central Punjab area, Pakistan, have been studied to characterize the
fasciolid populations involved. Morphometric analyses were made by applying
standardized measurements with a computer image analysis system (CIAS). The
current investigation is first time conducted in Pakistan to confirm the taxonomic
status of fasciolids by comparing with other pure standard populations viz., F.
hepatica of European Mediterranean origins and F. gigantica representing Burkina
Faso (Africa). In these geographical areas there is no overlapping of both fasciolids
species. Only parasites obtained from bovines were employed. The climatic factors
influencing fascioliasis presence and potential spread were analyzed from five
meteorological stations during 1990-2010. The fascioliasis forecast risk Mt and
Wb-bs (Water-Budget-Based System) indices and NDVI (Normalized Difference
Vegetation Index), known to be useful for fascioliasis assessment, were obtained
and correlated with geographical distribution, seasonality patterns and two-decade
evolution of fascioliasis in livestock throughout the province. These two climatic
forecast indices and a remote sensing marker are used to characterize the climatic
factors and the earth surface in order to ascertain the epidemiological complexity
and time-lag dynamics of fascioliasis. The seroprevalence of fascioliasis was also
determined in sub-tropical Punjab with the application of a very sensitive and
xxxispecific ELISA test by using monoclonal antibodies which are able to detect even
very low intensity infection. The MM3 Sero-ELISA was applied to check the status
of fascioliasis. The increase of disease transmission risk in the lowlands should be
highlighted, given that the largest part of the Punjab province includes low altitude,
highly irrigated plains. The importance of livestock in this province makes this
phenomenon to be given forecast priority assessment henceforth in order to
establish the adequate control measures. The use of cost effective diagnostic tools
would be helpful to reduce the impact of fascioliasis on animal health by selecting
the appropriate anthelmintic treatment. An annual treatment scheme to effectively
control the disease is finally recommended to be applied throughout the whole
Punjab province. |
en_US |
dc.description.sponsorship |
Higher Education Commission, Pakistan |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi Pakistan |
en_US |
dc.subject |
Natural Sciences |
en_US |
dc.title |
FASCIOLOSIS IN PAKISTAN: ADULT AND EGG PHENOTYPING, RISK MAPPING AND SEROLOGICAL STUDIES IN SUB-TROPICAL PUNJAB |
en_US |
dc.type |
Thesis |
en_US |