Abstract:
Amounts of DNA damage and homocysteine (Hcy) in heart patients blood may have strong function in the
causation of cardiovascular disease (CVD). The main objective of this work was to know experimentally the role of total
oxidants (produced by Reactive Oxygen species (ROS), clinical biochemical indices, their oxidized products and total
antioxidant status (TAS) among such patients to find the association of homocysteine, total oxidation status (TOS) and
oxidative DNA damage with other clinical parameters in sixty positive CVD patients compared with those of 60 normal
subjects. As compared to healthy individuals, CVD patients had significantly higher concentrations of homocysteine
(p<0.0001), total oxidants stress (TOS) (p<0.0001), serum total lipids (p<0.04), malondialdehyde (MDA) (p<0.001),
high density lipoprotein-cholesterol (HDL-C) (p<0.0001), and low density lipoprotein cholesterol (LDL-C) (p<0.01),
than those of healthy individuals. Plasma Hcy content, TOS and amount of DNA were positively and significantly
associated with cholesterol, triglycerides, systolic blood pressure, urea, and albumin (p values<0.01). TOS, Hcy and
oxidative DNA damage were negatively correlated with HDL-c, TAS and proteins. It is suggested that these parameters
have pivotal role in diagnostic process of determining severity in CAD patients. Oxidized products of macromolecules in
blood of CVD patients impart major functions in causing CVD disease.
