Abstract:
Caused by fungus, fungal keratitis is a kind of infections corneal disease with high rate of blindness, which
patients are mainly farmers in developing contries. Interleukin, as important proinflammatory cytokines, involve in
immune defense process against fungal infection of cornea. The expression of interleukin in the pathogenesis of fungal
keratitis, especially the main source of its cells, is not clear and the cell signaling pathways which regulate the synthesis
and modification of interleukin is still unknown. Caspase-11 was obtained and cultured. And the ELISA and Westernblot methods were used to explore the regulatory effect of Caspse-11 on Interleukin-1β in the fungal keratitis. neutrophils
were the main cell lineage of IL-1β to take part in the innate anti-fungi immunity in the cornea; IL-1β generation induced
by fungal infection might not be through the pre-excitation in the classical signal pathway; TLR4/TRIF pathway was not
involved in pro-IL-1β generation; while Dectin-1/syk pathway was involved in IL-1β generation in the fungal keratitis;
Caspase-l participated in the modification of IL-1β to change from the precursor into the mature body; but NLRP3
inflammasome and ASC inflammasome were not involved in IL-1β generation; Caspase-11 was involved in IL-1β
generation through regulating the modified process of Caspase-l to turning from precursor into mature body. TLR4/TRIF
pathway and NLRP3 inflammasome and ASC inflammasome are not involved in the pro-IL-1β generation, while
Caspase-l, Caspase-11 and Dectin-1/syk pathway are involved in the IL-1β generation
