Abstract:
The purpose of this study was to compare the polyphenol profile of different Rosemary (Rosmarinus
officinalis) extracts obtained from dry and fresh herb and the evaluation of their antioxidant effect. There were studied
the polyphenols from hydroalcoholic extracts (1:5- dry plant:solvent) obtained from fresh respectively dry plant and the
gemmotherapic extract obtained from fresh plant (1:20- dry plant: solvent). The polyphenol profile was evaluated by
UV-Vis spectrophotometry, TLC and HPLC. The total polyphenol respectively flavonoids content were determined by
UV-Vis spectrophotometry. The antioxidant effect was evaluated by DPPH, ABTS, FRAP, CUPRAC and silver
nanoparticle (SNP) methods. The hydroalcoholic extract obtained from fresh plant contains the highest concentration of
total polyphenols, (0.601mg/ml rosmarinic acid), total flavonoids, (0.270mg/ml luteoline) and rosmarinic acid (0.350
mg/ml). The less concentrated is the gemmotherapic extract, but also the extraction ratio is higher than the
hydroalcoholic extracts. The high content in polyphenols of the mentioned hydroalcoholic extract was confirmed by
highest values of antioxidant activity: 39.1ml (DPPH), 7.7 ml (ABTS), 698mM ET/100 ml (FRAP), 1947 mM ET/100
ml (CUPRAC), 4570mM ET/100 ml (SNP). These differences in the polyphenols profiles show the importance of use
the fresh plants for obtaining the good quality extracts
