Synergetic effects of GOS and Cu+2 nanoparticles as prebiotics on biochemical and metabolic hormonal profile in alloxan induced diabetic rats model

Abstract

Keeping in view the increased prevalence of diabetes and challenges in its effective treatment, current study was designed to assess the effect of new formulation {galactose oligosaccharide (prebiotic) and copper nanoparticles (np) (GOS/Cu2+)} on glycemic control, insulin levels, thyroid hormone (T3, T4) and oxidative stress in alloxan induced diabetic rats model. Normal healthy adult male rats (n=45) divided in three groups; healthy, diabetic control and diabetic treated groups with equal number of rats in each group (n=15). Diabetes was induced by intraperitoneal administration of alloxan at a dose of 120 mg/kg bw. Single oral dose of 200mg/kg bw/day was continued for 30 days to diabetic rats. Serum was separated from blood to measure glucose, insulin, thyroid hormone and oxidative stress level. Pancreatic tissue was collected in formalin (10%) to determine the activity of cellular reactive oxygen species. Treated group showed significantly (p<0.05) decreased level of glucose, malondialdehyde and cellular reactive oxygen species activity in pancreatic tissues; whereas significantly (p<0.05) increased level of insulin, thyroid hormones and catalase was seen in comparison to control. Conclusively, oral formulation of GOS/Cu+2 np have pronounced anti hyperglycemic as well as antioxidant effects that would be helpful to manage diabetic syndrome.