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Purification and characterization of β-galactosidase from Aspergillus fumigatus PCSIR-2013

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dc.contributor.author Bano, Saeeda
dc.contributor.author Iqbal, Samina
dc.contributor.author Siddiqui, Kausar
dc.contributor.author Abbasi, Kanwal
dc.date.accessioned 2022-10-18T10:45:32Z
dc.date.available 2022-10-18T10:45:32Z
dc.date.issued 2021-07-20
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13233
dc.description.abstract Extra cellular β-galactosidase enzyme was purified and characterized from Aspergillus fumigatus PCSIR2013. Estimated molecular mass of the enzyme was approximately 95 kDa. by native polyacrylamide gel electrophoresis. Initially, different fermentation parameters were optimized for maximum production of β-galactosidase. The kinetic study of the partially purified enzyme exhibited that it remained active in broad range of temperature from 25°C to 70°C with an optimum of 60°C. The Km and Vmax were calculated as 9.95mmol/l and 51.78 U/ml/min, respectively. The optimum pH was 5.0, when reaction mixture was incubated for 30 min. The enzyme was very stable in the presence of different metal ions, although Na+ (16%) stimulates the activity at 10mM concentration. In contrast, Ba+2 and Hg+2 have negative effect on enzyme activity and activity decreased to 54% and 19%, respectively. Thermo stability study was revealed that the enzyme retained 72% of its activity at 50°C. Whereas, when enzyme was incubated at 60°C for 120 min, its residual activity was decreased to 42.0%. However, the enzyme was completely inactivated at 80°C after 120 min of pre-incubation. Among different surfactant which incorporated with enzyme, Tween 20 and Triton X-100 both have stimulatory effect and activity increased to 29% and 17%, respectively. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciecnes, University of Karachi en_US
dc.subject Extra cellular β-galactosidase en_US
dc.subject enzyme activity en_US
dc.subject fermentation en_US
dc.subject chromatography en_US
dc.subject purification en_US
dc.title Purification and characterization of β-galactosidase from Aspergillus fumigatus PCSIR-2013 en_US
dc.type Article en_US


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