PASTIC Dspace Repository

Isolation, identification, and antimycotic activity of plumbagin from Plumbago europaea L. roots, leaves and stems

Show simple item record

dc.contributor.author Jaradat, Nidal
dc.contributor.author Khasat, Ahmad Ibrahim
dc.contributor.author Al-Maharik, Nawaf
dc.contributor.author M Eid, Ahmad
dc.contributor.author Jundi, Waheed
dc.contributor.author Zatar, Nidal Abd Aljaber
dc.contributor.author Shtayeh, Mohammed Saleem Ali
dc.contributor.author Jamous, Rana
dc.date.accessioned 2022-10-19T05:34:13Z
dc.date.available 2022-10-19T05:34:13Z
dc.date.issued 2021-07-20
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13260
dc.description.abstract Plumbago europaea L. is a plant utilized in Palestinian ethnomedicine for the treatment of various dermatological diseases. The current investigation was designed to isolate plumbagin from P. europaea leaves, roots and for the first time from the stems. Moreover, it aimed to evaluate the antimycotic activity against three human fungal pathogens causing dermatophytosis, also against an animal fungal pathogen. The qualitative analysis of plumbagin from the leaves, stems, and roots was conducted using HPLC and spectrophotometer techniques, while the structure of plumbagin was established utilizing Proton and Carbon-13 Nuclear Magnetic Resonance (NMR) and Infrared (IR) techniques. The entire plant constituents were determined by GC-MS. Moreover, the antimycotic activity against Ascosphaera apis, Microsporum canis, Trichophyton rubrum, and Trichophyton mentagrophytes was assessed utilizing the poison food technique method. The percentage of plumbagin recorded in the leaves, stems, and roots was found to be 0.51±0.001%, 0.16±0.001%, and 1.65±0.015%, respectively. The GC-MS examination declared the presence of 59 molecules in the plant extract. The plant extract and pure plumbagin exhibited complete inhibition against all tested dermatophytes at 6.0mg/mL for the extracts and 0.2mg/mL for plumbagin. P. europaea root is the best source of plumbagin and the plant extract could represent a potential drug candidate for the treatment of dermatophytosis infections. Further studies required to design suitable dosage forms from the natural P. europaea root extracts or plumbagin alone, to be utilized for the treatment of dermatological and veterinary ailments. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciecnes, University of Karachi en_US
dc.subject Antifungal en_US
dc.subject GC-MS en_US
dc.subject HPLC en_US
dc.subject NMR en_US
dc.subject plumbagin en_US
dc.subject Plumbago europaea en_US
dc.title Isolation, identification, and antimycotic activity of plumbagin from Plumbago europaea L. roots, leaves and stems en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account