Abstract:
The present study aimed to decipher the bacterial infections in diabetic foot human patients in Pakistan and the
anti-microbial susceptibility for clinical relevance. A total of 30 samples were collected from hospitalized type 2 diabetic
patients (men and women) having foot ulcers. The collected samples were cultured on mannitol salt agar, Blood agar and
MacConkey’s agar and cetrimide agar. Gram staining and specific biochemical tests were performed to identify the
invading bacteria. Antibiotic sensitivity and resistance pattern was performed for isolated bacteria by Kirby-Bauer disc
diffusion method. In diabetic foot ulcers, most prevalent bacteria were S. aureus with percent positivity of 83% followed
by E. coli (66%), K. pneumoniae (40%) and P. aeruginosa (16%). The infected ulcer with poly-microorganisms was
83.4% and the infected ulcer with single isolates was 16.6%. Imipenem was found to be most sensitive antibiotic against
Gram positive as well as Gram negative bacterial isolates from diabetic foot ulcer human patients. Gram negative
isolates from diabetic foot showed resistance to ampicillin, sulfamethoxazole/trimethoprim, cefotaxime/clavulanate,
metronidazole. The diabetic foot ulcers of human patients revealed high prevalence of S. aureus followed by E. coli, K.
pneumoniae and P. aeruginosa respectively. Imipenem was found to be the most sensitive antibiotic for all the bacterial
isolates from foot ulcers of type 2 diabetic patients. This study suggests imipenem as effective antibiotic for treatment of
diabetic foot ulcers against bacteria.
