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Assessment of acute, sub-acute, chronic and genotoxicity of polyherbal formulation DCD-684 in mice

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dc.contributor.author Roome, Talat
dc.contributor.author Qasim, Maha
dc.contributor.author Aziz, Sabahat
dc.contributor.author Farooq, Ahsana Dar
dc.contributor.author Razzaq, Anam
dc.contributor.author Ali, Syed Farooq
dc.date.accessioned 2022-10-19T06:01:14Z
dc.date.available 2022-10-19T06:01:14Z
dc.date.issued 2021-07-20
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13274
dc.description.abstract Digas colic drops (DCD-684) a polyherbal formulation containing Carum carvi, Foeniculum vulgare, Mentha arvensis, Mentha piperita and Zingiber officinale is widely used in Pakistan against gastrointestinal ailments including infantile colic. The DCD-684 (0.03-3ml/kg.bw) administered orally in acute (7-days) and sub-acute toxicity (14-days) tests, displayed neither mortality nor toxicological changes in physical, behavioral, biochemical and histopathological parameters. In chronic study (90-days), DCD-684 (0.3-12 ml/kg.bw) also revealed no changes. However, at 18 and 36 ml/kg.bw, liver demonstrated mild inflammation correlating with raised aspartate transaminase (AST), alkaline phosphatase (ALP) and alpha fetoprotein (AFP) levels. Increased levels of urea and inflamed renal parenchyma indicated mild nephro-toxicity with high alanine aminotransferase (ALT) at 36 ml/kg.bw. The LD50 of DCD-684 in mice was 27.5 ml/kg.bw. In hepatocytes at 36 ml/kg.bw, elevated mRNA expression of pro-inflammatory chemokines and cytokines were evident. DCD-684 neither damaged DNA nor induced cytotoxicity in micronucleus assay. In conclusion, polyherbal DCD-684 caused neither hepatic, renal, genotoxicity nor any undesirable effect in mice. Higher doses administered for 90 days showed mild toxic effects with no sign of necrosis, fibrosis or genotoxicity. Thus, in mice DCD-684 demonstrated a wide margin of safety to be used for the relief of infantile colic. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciecnes, University of Karachi en_US
dc.subject Digas colic drops en_US
dc.subject polyherbal formulation en_US
dc.subject chronic toxicity en_US
dc.subject genotoxicity en_US
dc.subject micronucleus assay en_US
dc.subject proinflammatory mRNA expression en_US
dc.title Assessment of acute, sub-acute, chronic and genotoxicity of polyherbal formulation DCD-684 in mice en_US
dc.type Article en_US


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