Abstract:
: Trianthema triquetra Rottl. ex Willed is being used as a herbal remedy for various diseases in India and
Pakistan. Still, no scientific data is available about therapeutic potential and phytochemistry of the plant. The aim of the
current investigation is to perform GC-MS analysis, antioxidant (total phenolic and flavonoid content, DPPH assay),
antimicrobial (disc diffusion assay) and cytotoxic (XTT and RBC’s cellular membrane protection assay) studies.
Methanolic extract and its fractions (n-hexane, ethyl-acetate, chloroform, n-butanol and water) were investigated for in
vitro studies. Results showed that n-butanol fraction exhibited a significant (p<0.05) antioxidant potential
(IC50=63.35±0.13 µg/mL) and also possess highest phenolic content (177±4.36 mg/g GAE). Whereas, n-hexane fraction
showed highest flavonoid content (14.67±1.53 mg/g QE). 2, 4-Ditert-butyl-6-nitrophenol (26.79%) and Squalene
(25.64%) were detected as major components through GC-MS analysis of chloroform fraction, eluted from column
chromatography. Moreover, chloroform fraction also exhibited antimicrobial potential. Significant (p<0.05) dose
dependent inhibition response on cell growth against CCRF-CEM cell lines was exhibited by methanolic extract.
Furthermore, hemolytic potential of methanolic extract was found to be in safe range (2.23%-6.37%). So, it can be
inferred that Trianthema triquetra can be exploited as an alternative remedy for cancer, oxidative stress related disorders
and various skin diseases.
