Abstract:
Given the substantial benefits of grape seed extract (GSE) in reducing oxidative stress, the study aimed
development, characterization and comparative analysis of GSE-based formulations. The development entailed
extraction of GSE from Vitisvinifera L. HPLC confirmed catechin, epicatechin, gallic acid, epicatechingallate and
procyanidin dimers. Storage of Formulations observed, Stability & rheological parameters determined. Olive oil used as
a permeability enhancer. Presence of the highest oleic acid content (65-86%) in Olive oil, skin permeability within the
stratum corneum was enhanced hence better transdermal skin absorption. Using two-way ANOVA, and T-test, efficacy
of formulations and impact on slowing down skin aging by countering exogenous factors of oxidative stress determined.
Non-invasive biophysical technique showed emulgel substantially reduced roughness, scaliness, winkles, and sebum
content by 55%, 26 %, 23.9% and 30.3% respectively enhancing elasticity and hydration by 50% and 32.2%
respectively. Emulsion reduced roughness, scaliness, winkles and sebum content 14%, 13%, 21% and 26.13%
respectively enhancing elasticity and hydration 45.3% and 29.85% respectively. The formulations significantly offset
exogenous factors of aging and impact on free radicals and oxidative stress and may be safe to incorporate bio-active
botanical antioxidants for evaluation of derma cosmetic benefits in management of dehydrated and aged facial skin.
