Abstract:
Red kidney beans have antioxidant effect and thereby can help in skin smoothening, moisturizing, whitening
and have anti-wrinkles effect. The study was based on the formulation of a stable w/o emulsion possessing extract of
Phaseolus vulgaris L. seeds, using paraffin oil with the aim to investigate its effect on various skin parameters. The
extract, achieved by concentrating ethanolic extract of red kidney beans was embedded in the internal aqueous part of
w/o emulsion. An active formulation possessing concentrated extract of red kidney beans and a placebo formulation
having no active material in the aqueous phase were formulated and placed at various conditions for the duration of 28
days, to observe the stability of cream. The placebo and formulation were stable at different storage conditions in terms
of phase separation and colour changes. Minute liquefaction was observed from 21stday up to 28th day in formulations
which were kept at 40°C +75% RH (relative humidity). With the passage of time significant changes were observed in
formulation pH while insignificant changes were observed at basic pH. Different effects of creams i.e placebo and
formulations were observed on the human skin by applying them on the volunteer’s cheeks for about 8 weeks. A stable
w/o emulsion can be formulated by using red kidney beans’ extract without any phase separation, liquefaction and colour
change over 28 days storage