Abstract:
Triptolide, an ingredient of Tripterygium wilfordii, has been demonstrated to possess many biological
activities such as immunomodulatory, antitumor activity in experiment. The purpose of this study was to survey the
toxicity of TPL-PEI-CyD on renal cells and its effects on breast carcinoma stem cells. The cytotoxicity of TPL-PEI-CyD
and TPL on HK-2 was comparatively assessed by CCK-8. After incubation and culturing with TGF-β1, the MCF-7 cells
were assessed by flow cytometry for the proportion of CD44
+ CD24
-
cells; then the CD44
+ CD24
-
cells were sorted by
immunomagnetic beads as MCF-7 stem cells. To assess the effect of TPL-PEI-CyD on MCF-7 stem cells, Western Blot
was used to detect the expression of Oct-4 and ALDHl in MCF-7 stem cells after being dosed with TPL- PEI-CyD.
Results showed that, compared with TPL, the toxicity of TPL-PEI-CyD on HK-2 cells was significantly reduced
(P<0.05). Breast carcinoma stem cells can be enriched by TGF-β1 and isolated from MCF-7 cells by immunomagnetic
sorting. TPL- PEI-CyD can even more significantly suppress the expression of Oct-4 and ALDHA1 in MCF-7 stem cells
than TPL (P<0.05). In conclusion, after coupling TPL and PEI-CyD, TPL-PEI-CyD showed characteristics of effective
suppression to breast carcinoma stem cell and decrease of cytotoxicity. It presented the unique effect of traditional
Chinese medicine as an efficient and low toxic drug carrier complex for breast carcinoma treatment.
