Abstract:
: In the present work, the antioxidant properties of methanolic (MeOH), ethyl acetate (EtOAc) and chloroformic
(CHCl3) fractions of Rosa damascena petals were evaluated. Antioxidant capacity was assessed by free radical
scavenging assays (DPPH•) and ferrous ions (Fe2+) chelating activity. Antibacterial activity was evaluated using
minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and IC50. Qualitative analysis of
chemical composition was carried out by HPLC and showed variability in the chemical constituents with a richness in
flavonones and phenolic acids. Acute toxicity study and hemolysis test were also assessed. The MeOH and EtOAc
fractions are of real and potential interest by their antioxidant activities. Furthermore, the microbiological study of the
fractions showed a high activity of the EtOAc fraction which possesses bactericidal properties, followed by a moderate
activity of the methanolic MeOH. The most sensitive strains were S. aureus and B. cereus while the most resistant were
P. aeruginosa and E. coli (R). On the other side, no cytotoxicity was observed towards erythrocytes isolated from human
blood and on a warm-blooded animal model. Therefore, the R. damascena petals constitute a promising source of
molecules for clinical use without cytoxicity.
