Abstract:
Diseases caused by oxidative stress can be prevented by antioxidant. Current treatments for those
neurodegenerative diseases are not effective and cause many side effects. Thus, the search for alternative medicines is in
high demand. Therefore, the main purposed of this study is to evaluate the neuroprotective effects of Curcuma longa
(rhizome) 80% methanol extract. Antioxidant using dichlorofuoresence diacetate (DCF-DA) assay on SH-SY5Y cells
revealed high activities of Curcuma longa (rhizome) extract with IC50 of 105.9±0.8 µg/mL. Sub-acute and chronic
toxicity tests of the plant extract on adult Javanese medaka (Oryzias javanicus) showed high toxicity effect with LC50 of
24.15±0.8 mg/L and 13.69±0.7 mg/L respectively. Neuroprotective tests using cholinesterase assay disclose significant
differences at P<0.05 between the group that are exposed to arsenic and treated with the crude extract and the group that
are exposed to only arsenic. Identification of vitexin and isovitexin justified the high antioxidant potential of this plant
leaf and it highest benefit to be used as medicinal supplement.
