Abstract:
Present work investigates the effects of hydro-methanolic roots extract (HyMREt) of Rauwolfia serpentina in
type 1 diabetic mice. Mice were divided into normal, diabetic, negative and positive controls (I-IV) and three test
(HyMREt doses) groups (V-VII - 50, 100, &150mg/kg). Allocated treatment of each group was given orally for 14 days
in overnight fasted state. Percent change in fasting blood glucose (FBG), body weights, body tissue weights, hepatic
glycogen, total lipids, glycosylated hemoglobin (HbA1c), complete blood profile and antioxidant enzymes including
catalase (CAT) and superoxide dismutase (SOD) were estimated. HyMREt doses produced meaningful (p<0.0001)
reduction (-39 to -53%) in FBG. Hemoglobin (Hb) levels were raised, HbA1c were considerably decreased (4.5-3.77%)
and glycosylation (HbA1c to Hb) ratio was expressively (p<0.0001) improved in test groups. Dose-wise improvement
(p< 0.05) in total glycogen and decrement (p<0.05) in lipids were observed in livers of test groups. HyMREt
significantly decreased (p<0.05) percent inhibition of SOD and CAT. HyMREt doses progressively (p<0.05) improved
RBC and other hematological parameters while decrement was only noticed in leucocyte counts. Administration of test
doses of HyMREt were significantly reduced the glycosylation, oxidative stress and anemia caused by alloxan
intoxication in mice.
