Abstract:
Inflammation is an aggregate of different pathologic responses in body that leads to life threatening conditions
if not combated at early stages. A variety of chemical medications from low quality to high quality are available in
market for treatment of inflammation. However the side effects posed by these medications cannot be ignored. Here in
our study we have shown for the first time, the anti-inflammatory effects of SBF compound that is obtained from wild
mushroom species that are Acremonium sp. HKI 0230 and Coprinus echinosporus. We employed Nitric oxide
determination, cell viability assay, RT-PCR and western blot analysis to check the anti-inflammatory effects of SBF. The
antioxidant activity of this compound has been studied in detail in past, but our results have shown that SBF potently
suppressed the Nitric oxide production (NO) without any cytotoxicity to the model cell line; RAW 264.7 cells. It also
inhibited the production of major proinflammatory mediators and cytokines i.e. iNOS, COX-2, IL-1β, IL-6 and TNF-α.
SBF elicited its anti-inflammatory effects via the canonical NF-κB and MAPK pathway. Taken together, our results have
shown that SBF exhibits excellent anti-inflammatory activity in vitro and further experimentations may warrant its
application as a commercial herbal remedy for inflammation related anomalies.
