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Simvastatin inhibites high glucose-induced renal tubular epithelial cells apoptosis by down-regulating miR-92a

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dc.contributor.author Guan, Ying
dc.contributor.author Zhou, Ping
dc.contributor.author Sun, Zhangsong
dc.contributor.author Meng, Lifeng
dc.date.accessioned 2022-10-20T10:06:53Z
dc.date.available 2022-10-20T10:06:53Z
dc.date.issued 2021-01-16
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13467
dc.description.abstract This work aims to investigate the role of simvastatin (SIM) in renal tubular epithelial cells (HK-2) proliferation. The apoptosis model of HK-2 cells induced by high glucose was established; HK-2 cells were cultured in vitro and randomly divided into control group, model group, SIM low-dose group, SIM medium-dose group and SIM high-dose group. After 24 h culture, the inhibitory effect of SIM on high glucose- induced proliferation of HK-2 cells was evaluated by MTT method. The expression of cysteinyl aspartate specific proteinase (Caspase-3) in apoptosisrelated protein was evaluated by Western blotting; miR-92a expression in HK-2 cells was measured by RT-qPCR. High glucose group had significantly lower HK-2 cell survival rate than the control group (p<0.05); SIM middle-dose and high-dose groups had higher HK-2 cell survival rate than the model group, (p<0.05); SIM low, medium and high-dose groups had lower HK-2 cell apoptosis rate, Caspase-3 protein and miR-92a expression levels than the model group (p<0.05), all showing dose-dependence. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciecnes, University of Karachi en_US
dc.subject Simvastatin en_US
dc.subject miR-92a en_US
dc.subject renal tubular epithelial cells en_US
dc.subject apoptosis en_US
dc.title Simvastatin inhibites high glucose-induced renal tubular epithelial cells apoptosis by down-regulating miR-92a en_US
dc.type Article en_US


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