EVALUATION OF COPPER NANOPARTICLE IN TISSUE CULTURE MEDIA FOR CONTROLLING BIO CONTAMINATION

Abstract

The present study was aim to examine effect of Copper nanoparticles on biological contamination in tissue culture of Coriandum sativum. This herbaceous plant belongs to family Umbelliferae, which is highly reputed ayurvedic medicinal herb commonly known as the Dhanya. The sterilized and certified seeds of C. sativum were grown in petri plates which were use as explants for Tissue Culture. The MS medium supplemented with different PGRs in separate and combination was used. The best callogenesis was obtained in MS medium with 35g/L of sucrose, 2,4,D+BAP at 5.7 pH. The temperature was optimized best at 25ᵒC. The synergetic effect of 2,4,D+BAP resulted in the best production of 88% whitish yellow callus after 21 days of inoculation. The Copper sulphate salt was used for the formation of copper nanoparticles from the leaves of C sativum. The nano particles prepared from water and ethanol were added in the MS medium. The Copper nano particles (ethanol) have shown dynamic effect on controlling biological Contamination (Fungal and Bacterial growth) at 5 mg/L of MS medium. The confirmation of Cu nano particle was done by Ultra Violet Spectrometry (UV) and Fourier Transform Infrared Spectroscopy (FTIR). The UV-Vis spectroscopy Absorption Spectra of Cu nano particle had peak range of 300 nm and 600 nm respectively. The FTIR results of Cu Nano particle showed the presence of bending and stretching vibration of biomolecules. We can claim that suggested that Copper nano particle can decrease and remove microorganisms in MS media and then the explants can growth very well. This was the first repost of use of Cu nanoparticle as an antimicrobial agent in tissue culture of Coriandum sativum.