Abstract:
The present study was design to produce nanoparticles from callus of Raphanus sativus using different explant (leaf and node). This plant belongs to the family Brassicaceae and is used worldwide as a vegetable, animal fodder, and for various oil productions. Different Plant Growth Regulators were used (separate and combination) including 2,4-D and BAP. The physical and chemical factor were optimized best at pH 5.7, Temperature 25˚C, photoperiod 16 hours and best concentration of sucrose 30g/L in solid medium. The best percentage of callogenesis (90%) was obtained with combination of 3.5mg/l 2,4 D and 3.5mg/l BAP. For making a comparison for in vivo and in vitro Silver nanoparticle, the leaves & callus of Raphanus sativus were used for their biosynthesis. Two solvents were used (Water and Ethanol). The best nanoparticles were obtained from water with AgNO3 by the callus of Raphanus sativus. The characterization and evaluation of Prepared Nano particle were done by FTIR and UV Spectroscopy for confirmation. The UV-Vis spectroscopy results showed that the Absorption Spectra of Silver Nanoparticles from leaf and callus extracts had absorbance peak range of 300 nm and 600 nm respectively. The FTIR results of Silver Nano particle showed the presence of some important biomolecules in callus extracts that act as reducing and capping agent. This work is the first report on synthesis of silver nanoparticles from Raphanus sativus calli. This study may help to improve callogenesis from protocols and nanoparticles formation.
