Abstract:
Based on the ethnomedicinal use of Isodon rugosus the current study was designed to evaluate its crude
saponins (Ir.Sp), and subsequent fractions for anti-angiogenic and anti-tumor potentials. Chorioallantoic membrane
(CAM) assay was used in anti-angiogenic potentials with Dexamethasone as positive control. The antitumor activity was
evaluated with potato disk method using Vincristine sulfate as positive control. Moreover, antibacterial activity was also
conducted against A. tumefaciens. The highest anti-angiogenic effect was observed with Ir.Sp, i.e., 79.00±0.58% at
concentration of 1000 µg/ml which drop drown to 48.67±1.20% at lowest tested concentration of 31.25 µg/ml with IC50
of 41 µg/ml. Similarly, in the anti-tumor activity the Ir. Chf revealed excellent inhibition of tumor with IC50 value of 60
µg/ml. All the samples (excluding Ir. Sp and Ir. Cr) were inactive against A. tumefaciens, which demonstrates that the
samples which did not show any antibacterial activity are rich in certain bioactive principles which may be responsible
for the anti-tumor and anti-angiogenic potentials. Our results conclude that the Ir.Sp, Ir.Chfmay be good targets for
isolation of bioactive compounds responsible for the inhibition of excessive proliferation of cells and angiogenesis
