Abstract:
The purpose of the studies was to evaluate an in-vitro anti-mycobacterial activity of Aloe vera and Allium
sativum against MDR-MTB, their cytotoxicity and mutagenicity. Four extracts of Aloe vera and Allium sativum were
prepared by Soxhlet apparatus and their minimum inhibitory concentrations (MIC’s) were determined by BACTEC
MGIT960 system against multi drug resistant Mycobacterium tuberculosis (MDR-MTB) isolates, collected from
pediatric patients. Fractions of Aloe vera and Allium sativum extracts were separated using glass column
chromatography, followed by evaluation of cytotoxicity and mutagenicity by tetrazolium salt (3-(4,5-dimethylthiazol-2-
yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Ames test, respectively. Out of four extracts, ethanol extracts of
Aloe vera and Allium sativum exhibited activity at MIC 5mg/mL to 7mg/mL and 3mg/mL to 5mg/mL, respectively and
IC50 by MTT assay for combination of all fractions were 278.3mcg/100µL and 270.8mcg/100µL and in Ames assay M.I
of TA98 were 0.14 and 0.07 and M.I of TA100 were 1.14 and 0.44, respectively. Aloe vera and Allium sativum extracts
showed anti-mycobacterial activity against MDR-MTB isolates so, MIC of ethanol extracts of each plant and fractions of
column chromatography had been checked. The MTT and Ames tests depicted that ethanol extracts of Aloe vera and
Allium sativum were non-cytotoxic and non-mutagenic, and can be used in treatment of patients suffering from MDRMTB.
