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Appraisal of anti-mycobacterial potential against MDR-MTB in pediatric patients, cytotoxicity and mutagenicity of Aloe vera and Allium sativum

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dc.contributor.author Iqbal, Aqeela
dc.contributor.author Ashraf, Muhammad
dc.contributor.author Rathore, Ahsan Waheed
dc.contributor.author Omer, Muhammad Ovais
dc.contributor.author Anjum, Aftab Ahmad
dc.contributor.author Bano, Iqbal
dc.contributor.author Ali, Muhammad Asad
dc.contributor.author Ali, Rana Adnan
dc.contributor.author Hafeez Ullah
dc.contributor.author Zaman, Muhammad
dc.date.accessioned 2022-10-24T08:39:59Z
dc.date.available 2022-10-24T08:39:59Z
dc.date.issued 2021-01-16
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13576
dc.description.abstract The purpose of the studies was to evaluate an in-vitro anti-mycobacterial activity of Aloe vera and Allium sativum against MDR-MTB, their cytotoxicity and mutagenicity. Four extracts of Aloe vera and Allium sativum were prepared by Soxhlet apparatus and their minimum inhibitory concentrations (MIC’s) were determined by BACTEC MGIT960 system against multi drug resistant Mycobacterium tuberculosis (MDR-MTB) isolates, collected from pediatric patients. Fractions of Aloe vera and Allium sativum extracts were separated using glass column chromatography, followed by evaluation of cytotoxicity and mutagenicity by tetrazolium salt (3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Ames test, respectively. Out of four extracts, ethanol extracts of Aloe vera and Allium sativum exhibited activity at MIC 5mg/mL to 7mg/mL and 3mg/mL to 5mg/mL, respectively and IC50 by MTT assay for combination of all fractions were 278.3mcg/100µL and 270.8mcg/100µL and in Ames assay M.I of TA98 were 0.14 and 0.07 and M.I of TA100 were 1.14 and 0.44, respectively. Aloe vera and Allium sativum extracts showed anti-mycobacterial activity against MDR-MTB isolates so, MIC of ethanol extracts of each plant and fractions of column chromatography had been checked. The MTT and Ames tests depicted that ethanol extracts of Aloe vera and Allium sativum were non-cytotoxic and non-mutagenic, and can be used in treatment of patients suffering from MDRMTB. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciecnes, University of Karachi en_US
dc.subject Allium sativum en_US
dc.subject Aloe vera en_US
dc.subject MDR-MTB en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject tuberculosis en_US
dc.subject TB in children en_US
dc.title Appraisal of anti-mycobacterial potential against MDR-MTB in pediatric patients, cytotoxicity and mutagenicity of Aloe vera and Allium sativum en_US
dc.type Article en_US


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