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AUXIN EFFECTS ON SOMACLONAL VARIATION AND PLANT REGENERATION FROM MATURE EMBRYO OF BARLEY (HORDEUM VULGARE L.)

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dc.contributor.author AYDIN, MURAT
dc.contributor.author TASPINAR, SINAN
dc.contributor.author ARSLAN, ESRA
dc.contributor.author SIGMAZ, BURCU
dc.contributor.author AGAR, GULERAY
dc.date.accessioned 2022-10-24T11:13:28Z
dc.date.available 2022-10-24T11:13:28Z
dc.date.issued 2015-10-20
dc.identifier.citation Aydın, M., Taspınar, M. S., Arslan, E., Sigmaz, B., & Agar, G. (2015). Auxin effects on somaclonal variation and plant regeneration from mature embryo of barley (Hordeum vulgare L.). Pakistan Journal of Botany, 47(5), 1749-1757. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13609
dc.description.abstract Crop improvement through genetic engineering depends on effective and reproducible plant regeneration systems. In barley (Hordeum vulgare L.), immature embryos are the most commonly used as explant source for In vitro regeneration and genetic transformation but, mature embryos are alternative to immature embryo due to the fact that they have advantages such as easy storage and ready availability throughout the year. The effects of different concentrations (2, 4, 6, 8, 10 and 12 mg/l) of three auxins (2,4-D, dicamba and picloram) were evaluated in mature embryo culture of barley. Calli, embryogenic calli and regenerated plants were observed in all of the studied auxins. The MS (Murashige & Skoog basal medium) containing 12 mg/l dicamba was found to be the most effective for embryogenic callus, responded embryogenic callus and regeneration efficiency. Plant tissue culture can bring about genetic changes that are known as somaclonal variation. Genetic and epigenetic changes were examined by RAPD (Randomly Amplified Polymorphic DNA) and CREDRA (Coupled Restriction Enzyme Digestion-Random Amplification) techniques, respectively. Increased auxin concentration led to the decrease in the GTS (Genomic Template Stability) value. DNA hypermethylation occurred in higher concentrations of 2,4-D and picloram, while DNA hypomethylation was observed in dicamba. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society, University of Karachi en_US
dc.subject Barley en_US
dc.subject RAPD en_US
dc.subject CRED-RA 4-D en_US
dc.subject Dicamba, Picloram en_US
dc.title AUXIN EFFECTS ON SOMACLONAL VARIATION AND PLANT REGENERATION FROM MATURE EMBRYO OF BARLEY (HORDEUM VULGARE L.) en_US
dc.type Article en_US


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