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AN IMPROVED, SIMPLE, INEXPENSIVE AND HIGHLY FLEXIBLE HYDROPONIC SETUP FOR ROOT MITOCHONDRIA ISOLATION FROM ARABIDOPSIS AND NICOTIANA PANTS

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dc.contributor.author WAQAR HAMEED, MUHAMMAD
dc.contributor.author UD-DIN, AZIZ
dc.date.accessioned 2022-10-24T11:28:56Z
dc.date.available 2022-10-24T11:28:56Z
dc.date.issued 2015-11-22
dc.identifier.citation Hameed, M. W. (2015). An improved, simple, inexpensive and highly flexible hydroponic setup for root mitochondria isolation from Arabidopsis and Nicotiana pants. Pakistan Journal of Botany, 47(5), 2037-2043. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13643
dc.description.abstract Hydroponic setups are frequently developed and improved as they are convenient platforms for studying whole plant physiology. Mostly, the available systems produce small amounts of plant material and are therefore, unsuitable for studies requiring large quantities of plant material like isolation of mitochondria. To address this issue, we have modified a hydroponic setup that can sustain hundreds of Arabidopsis and tobacco plants until adult plants are established. The setup is very flexible and easy to construct. It is based on the use of recyclable and sterilizable plastic-net-pots and media containers, which are easily available from the local suppliers. The modified seed-pots and styrofoam sheets facilitate the transfer and harvesting of seedlings. We have used the Percoll based two-step density gradient centrifugation method for the isolation of root mitochondria from the hydroponically grown plants. en_US
dc.language.iso en en_US
dc.publisher Karachi:Pakistn Botanical Society, Karachi en_US
dc.subject Hydroponic en_US
dc.subject Arabidopsis en_US
dc.subject Nicotiana en_US
dc.subject Roots en_US
dc.subject Mitochondria isolation en_US
dc.title AN IMPROVED, SIMPLE, INEXPENSIVE AND HIGHLY FLEXIBLE HYDROPONIC SETUP FOR ROOT MITOCHONDRIA ISOLATION FROM ARABIDOPSIS AND NICOTIANA PANTS en_US
dc.type Article en_US


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