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IN SILICO BASED DEVELOPMENT OF dLUTE LENGTH POLYMORPHISM MARKER FOR COMMON FLAX GERMPLASM EVALUATION

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dc.contributor.author ŽIAROVSKÁ, JANA
dc.contributor.author BEŽO, MILAN
dc.contributor.author LANCÍKOVÁ, VERONIKA
dc.contributor.author RAŽNÁ, KATARÍNA
dc.date.accessioned 2022-10-24T11:30:00Z
dc.date.available 2022-10-24T11:30:00Z
dc.date.issued 2015-11-24
dc.identifier.citation Žiarovská, J. A. N. A., Bežo, M. I. L. A. N., Lancikova, V., & Ražná, K. A. T. A. R. Í. N. A. (2015). In silico based development of dLUTE length polymorphism marker for common flax germplasm evaluation. Pak. J. Bot, 47(5), 2277-2282. en_US
dc.identifier.issn 2070-3368
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13645
dc.description.abstract The first study of the pattern of dLUTE insertions as detected by PCR length polymorphism technique was performed. In silico approach was used for developing the dLUTE based marker system. Alignment analysis were performed to find a region that is dLUTE specific and primers designed withing this region not corresponding to any of sequences of L-genes. Realized length polymorphism analysis of common flax accessions confirmed the results of In silico analysis of polymorphic potential of designed marker system. The PCR analyse based of flax dLUTE transposon resulted in ten different alleles amplified in PCR withing the length range from 250 up to the 980 bp. In total, twenty-four common flax accessions were evaluated using the developed approach and the grouping of them according the oil/fiber type was obtained in constructed dendrogram. en_US
dc.language.iso en en_US
dc.publisher Karachi:Pakistn Botanical Society, Karachi en_US
dc.subject Linum usitatissimum L en_US
dc.subject dLUTE en_US
dc.subject Length polymorphism en_US
dc.subject DNA marker en_US
dc.title IN SILICO BASED DEVELOPMENT OF dLUTE LENGTH POLYMORPHISM MARKER FOR COMMON FLAX GERMPLASM EVALUATION en_US
dc.type Article en_US


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