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Relaxant activity of different fractions of fruits of Rosa moschata J.

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dc.contributor.author Nabi, Muhammad
dc.contributor.author Ali, Niaz
dc.contributor.author Shams, Bilal
dc.contributor.author Ahmed, Ghayour
dc.contributor.author Shah, Muhammad Jalat
dc.contributor.author Shakir Ullah
dc.contributor.author Shoaib, Mohammad
dc.contributor.author Shah, Syed Wadood Ali
dc.date.accessioned 2022-10-26T04:00:58Z
dc.date.available 2022-10-26T04:00:58Z
dc.date.issued 2020-03-16
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13667
dc.description.abstract The current work is an attempt to know that in which fraction(s) the relaxant constituents of Rosa moschata concentrate. Crude methanolic extract of Rosa moschata was prepared as per our reported procedure. Sub fractions of methanol extract were extracted with different solvents in increasing order of polarity i.e. n-hexane > chloroform > ethyl acetate > n-butanol > residual aqueous fractions. Different concentrations (0.01, 0.03, 0.1, 0.3, 1, 3, 5 and 10 mg/ml) of the fractions were tested on spontaneous contractions and KCl induced contractions on rabbits’ jejunal preparations. Calcium Concentration Response Curves (CCRCs) in the presence and absence of the test fractions using verapamil were constructed to understand its mechanisms. EtOA fraction was more relaxant with EC50 values 0.812±0.149 mg/ml on spontaneous and 2.01±0.08 mg/ml on KCl induced contractions. we also found right shift in its EC50 values expressed as log [Ca++]M values. In presence of 0.3 mg/ml EtOA fraction, its EC50 value was -2.22±0.035 vs control EC50 -2.71±0.21. For n-BuOH fraction, EC50 value was -1.82±0.00 vs control with EC50 -2.28±0.049 at concentration of 0.3 mg/ml. Ethyl acetate fraction of Rosa moschata was more potent and is therefore can be a target for activity guided isolation of calcium channel antagonists. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciecnes, University of Karachi en_US
dc.subject Rosa moschata J. en_US
dc.subject fractions of Rosa moschata en_US
dc.subject verapamil en_US
dc.subject calcium channel blocking en_US
dc.subject relaxant activity en_US
dc.title Relaxant activity of different fractions of fruits of Rosa moschata J. en_US
dc.type Article en_US


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