Effect of Monosodium Glutamate on Wall thickness of Fallopian tubes of Albino Rats

Abstract

Background: Monosodium Glutamate is a mono sodium salt of a naturally occurring amino acid i.e. glutamic acid. It gives meaty taste thus improves food palatability & appetite. Aim: To evaluate the effects of MSG given to the albino rats on morphology & histology of fallopian tubes. Methods: Sample size of 45 female adult albino rats was taken i.e. 15 animals in each of three groups; control group (A), low dose experimental group (B) and high dose experimental group (C). Animals of group B were given a dose of 0.04 mg/kg body weight of MSG after dissolving in 1 ml of distilled water for 14 days through orogastric tube and that of group C were given a dose of 0.08 mg/kg body weight after dissolving in 1 ml of distilled water for equal days. Group A animals received only equal amount of distilled water without any salt for same days. Dissection was made on the fifteenth day of the experiment of all animals. Abdomens were opened and fallopian tubes were identified. They were cleared from surrounding tissues and dissected out. Gross examination of each was done for any change in appearance. They were then weighed and their lengths were taken. Proper fixation of tissue was done and then 5µm serial sections of tissues were made. Hematoxylin/eosin staining was performed for detailed histological study of the tissue. Wall thickness of each tube was measured from three sites by ocular micrometery. Results: Wall thickness of Group B (low dose experimental) was more than Group C (high dose experimental). Conclusion: MSG when given orally for short period of time causes effects on fallopian tubes so its use for longer time can cause severe effects