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Antitumor activity of a novel survivin siRNA

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dc.contributor.author Li, Yuhuan
dc.contributor.author Li, Yujing
dc.contributor.author J Lee, Robert
dc.contributor.author Wang, Xi
dc.contributor.author Du, Lin
dc.contributor.author Zhao, Lingzhi
dc.contributor.author Liu, Yan
dc.contributor.author Teng, Lesheng
dc.date.accessioned 2022-10-28T08:16:00Z
dc.date.available 2022-10-28T08:16:00Z
dc.date.issued 2015-09-15
dc.identifier.citation Li, Y., Li, Y., Lee, R. J., Wang, X., Du, L., Zhao, L., ... & Teng, L. (2015). Antitumor activity of a novel survivin siRNA. Pakistan Journal of Pharmaceutical Sciences, 28. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13879
dc.description.abstract Breast cancer resistance to therapy can result from expression of antiapoptotic genes. Survivin is an antiapoptotic gene that is over expressed in most human tumors. RNA interference using short interfering RNA (siRNA) can be used to specifically inhibit survivin expression. A novel siRNA targeting survivin was used to process MCF-7 cells. Cellular survivin mRNA and protein levels were determined by real-time qRT-PCR and Western blot, respectively. Cellular morphology and cell cycle were determined by fluorescence microscopy and flow cytometry. Cell proliferation was measured by MTT assay. Our data showed that the novel survivin-targeted siRNA could efficiently knockdown the expression of survivin, inhibit cell proliferation and cell cycle, especially at the G2/M checkpoint. These data suggest that the siRNA has potential for therapeutic applications. en_US
dc.language.iso en en_US
dc.publisher Karachi:Pakistan Journal of Pharmaceutical Sciences, university of Karachi. en_US
dc.subject Survivin en_US
dc.subject siRNA en_US
dc.subject breast cancer en_US
dc.subject cell proliferation en_US
dc.subject cell cycle en_US
dc.title Antitumor activity of a novel survivin siRNA en_US
dc.type Article en_US


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