Abstract:
Curcumin (Diferuloylmethane) is a natural product extracted from the root of Curcuma longa. 5-Bis (4-
hydroxy-3-methoxybenzylidene)-N-methyl-4-piperidone, the piperidone analogue of curcumin (PAC), was one of the
analogues that, demonstrated potential anticancer effects against breast and colon cancers compared with native
curcumin. A simple, accurate, and rapid isocratic reverse phase high performance liquid chromatography (HPLC)
analytical method utilizing UV detection was developed and validated for the determination of PAC utilizing C18 column
with run time was 7 min. Chromatogram showed a peak of PAC at retention time of 5.8±0.92 min. The method was
validated for linearity, accuracy, precision, limit of detection, limit of quantitation and robustness. Linear relationship (r
> 0.99) was observed between AUP of PAC and the corresponding concentrations over 100-10000µg/mL. The LOQ of
this assay was 3.9ng/mL with a corresponding relative standard deviation of 4.8 and 4.0%. The LOD was 13.1ng/mL at a
signal-to-noise ratio of >3
