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A validated RP-HPLC method for the determination of piperidone analogue of curcumin

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dc.contributor.author Alomrani, Abdullah
dc.contributor.author Alhabardi, Samiah
dc.contributor.author Aboussekhra, Abdelilah
dc.contributor.author Alshamsan, Aws
dc.contributor.author Attia, Mohamed I
dc.contributor.author Al Quadeib, Bushra
dc.date.accessioned 2022-10-28T08:17:56Z
dc.date.available 2022-10-28T08:17:56Z
dc.date.issued 2020-03-16
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13887
dc.description.abstract Curcumin (Diferuloylmethane) is a natural product extracted from the root of Curcuma longa. 5-Bis (4- hydroxy-3-methoxybenzylidene)-N-methyl-4-piperidone, the piperidone analogue of curcumin (PAC), was one of the analogues that, demonstrated potential anticancer effects against breast and colon cancers compared with native curcumin. A simple, accurate, and rapid isocratic reverse phase high performance liquid chromatography (HPLC) analytical method utilizing UV detection was developed and validated for the determination of PAC utilizing C18 column with run time was 7 min. Chromatogram showed a peak of PAC at retention time of 5.8±0.92 min. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantitation and robustness. Linear relationship (r > 0.99) was observed between AUP of PAC and the corresponding concentrations over 100-10000µg/mL. The LOQ of this assay was 3.9ng/mL with a corresponding relative standard deviation of 4.8 and 4.0%. The LOD was 13.1ng/mL at a signal-to-noise ratio of >3 en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciecnes, University of Karachi en_US
dc.subject Piperidone analogue of curcumin en_US
dc.subject reverse phase high performance liquid chromatography en_US
dc.title A validated RP-HPLC method for the determination of piperidone analogue of curcumin en_US
dc.type Article en_US


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