Abstract:
The present work reports the synthesis of Schiff base series of nicotinic hydrazide (C-1-C-5) and it’s
antibacterial and wound healing evaluation. The synthetic molecules were characterized with different spectroscopic
techniques and explored for their antibacterial potential. The objective of this work was to explore antimicrobial agent
using two types of microorganisms, one Gram-positive (S. aureus ATCC 9144) and one Gram-negative (E. coli ATCC
10536). C-2, C-4 and C-5 potentially inhibit bacterial growth (p<0.001). Atomic force microscopy (AFM) imaging was
obtained to get high-resolution images of the effect of treated drugs on the bacterial morphology. The images obtained
also revealed the antibacterial effects of potent molecule. The magnified pictures captured under AFM suggest
significantly damaged cell surface and disturbed morphology. The compounds were further analyzed for in vivo wound
healing potential on mice. The compound C-2, C-4 and C-5 heal the wounds comparatively in less time duration as
compared to control group (p<0.001). Compound C-1 and C-3 took more time to heal the wound as compare to
compound C-2, C-4 and C-5. The re-epithelialization process of wound in animals group treated with potent compound
was highly significant (p<0.001) and faster than control. Results of this study suggest that the compounds C-2, C-4
andC-5 possess pronounced antibacterial and wound healing potential and need to be further evaluated for mechanism of
action.
