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Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes

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dc.contributor.author Neumüller, Jasna Bošnjak
dc.contributor.author Radaković, Milena
dc.contributor.author Djelić, Ninoslav
dc.contributor.author Gačić, Branka Vuković
dc.contributor.author Stevanović, Zora Dajić
dc.contributor.author Kolarević, Stoimir
dc.contributor.author Mišić, Danijela
dc.contributor.author Stanković, Milan
dc.contributor.author Vukčević, Jelena Knežević
dc.contributor.author Potparević, Biljana Spremo
dc.contributor.author Stanimirović, Zoran
dc.date.accessioned 2022-11-03T10:23:51Z
dc.date.available 2022-11-03T10:23:51Z
dc.date.issued 2017-03
dc.identifier.citation Bošnjak-Neumüller, J., Radaković, M., Djelić, N., Vuković-Gačić, B., Stevanović, Z. D., Kolarević, S., ... & Stanimirović, Z. (2017). Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes. Pakistan journal of pharmaceutical sciences, 30. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/13935
dc.description.abstract The success of antioxidant therapy in hyperthyroidism implies that disease is mediated by oxidative stress, which is known as one of the causing agents of ageing, degenerative diseases, and cancer. The main objective of our study was to determine possible protective effects of methanolic extract of N. rtanjensis in triiodothyronine (T3)-induced DNA breaks of human lymphocytes under in vitro conditions, based upon plant antioxidant capacity related to its phytochemical profile, mainly its polyphenolic complex. The total phenolic and flavonoid content and the antioxidant activity using in vitro 1,1-dyphenyl-2- picrylhydrazyl reagent (DPPH) was determined in methanolic extracts of plant leaves and flowers. The phenolic compound content of 62.73±1.80mg of GaA/g, exhibited solid antioxidant activity (IC50= 112.59±0.95μg/ml). The antigenotoxic activity of 0.2, 0.5 and 1.0mg/ml N. rtanjensis methanol extracts mixture with 100μM of T3 was studied in human lymphocytes in vitro using the Comet assay. It is supposed that the antigenotoxicity of N. rtanjensis methanol extracts was caused by high presence of chlorogenic acid, rosmarinic acid and rutin, all known as efficient antioxidant bioactive compounds, which were determined by ultrahigh-pressure liquid chromatograph with MS/MS Mass Spectroscopy (UHPLC/-HESI-MS / MS). en_US
dc.language.iso en_US en_US
dc.publisher Faculty of Pharmacy & Pharmaceutical Sciences en_US
dc.subject triiodothyronine en_US
dc.subject DNA damage en_US
dc.subject Nepeta rtanjensis en_US
dc.subject phenolic compounds en_US
dc.subject antigenotoxic activity en_US
dc.title Nepeta rtanjensis (Lamiaceae), a plant endemic to the Balkans: Phenolic composition, antioxidant activity, and in vitro antigenotoxic effects in triiodothyronine-induced DNA damage in human lymphocytes en_US
dc.type Article en_US


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