Abstract:
In order to investigate the mechanisms of nitrogen (N) re-distribution in response to proteolytic enzyme (PE),
glutamine synthetase (GS) and glutamate synthetase (GOGAT) activities and the effects on N use efficiency (NUE). Two oilseed rape genotypes were grown in sand culture in a greenhouse under normal (15.0 mmol L-1 NO3 -) and limited-N (7.5 mmol L-1 NO3 -) levels. Isotope (15N) labeling and enzyme inhibitors against the PE, GS and GOGAT enzymes were used. We found that, when the two genotypes were subjected to specific inhibitors of PE, GS, and GOGAT, the activities of these enzymes were significantly decreased, resulting in reduced N re-distributed from leaf to grain, as well as reduced NUE. Lglutamine and free amino acid contents in the phloem sap were primarily influenced by PE and GS activities, whereas grain yield was primary regulated by GOGAT activity during the later growth stages. These findings suggest that PE, GS, and GOGAT are key enzymes for the regulation of N re-distribution in plant tissues during later growth stages, with grain yield
and NUE of oilseed rape being positively regulated by PE, GS and GOGAT activities.
