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Development and validation of HPLC method for the determination of Cefpodoxime Proxetil in human plasma

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dc.contributor.author Bashir, Lubna
dc.contributor.author Harris Shoaib, Muhammad
dc.contributor.author Israr, Fozia
dc.contributor.author Siddiqui, Fahad
dc.date.accessioned 2022-11-18T07:10:20Z
dc.date.available 2022-11-18T07:10:20Z
dc.date.issued 2017-09-12
dc.identifier.citation Bashir, L., Shoaib, M. H., Naz, S., Yousuf, R. I., Jabeen, S., Israr, F., & Siddiqui, F. (2017). Development and validation of HPLC method for the determination of cefpodoxime proxetil in human plasma. Pak J Pharm Sci, 30, 1603-7. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14072
dc.description.abstract A new, simple, accurate, precise and specific method has been developed for the analysis of Cefpodoxime Proxetil in human plasma. The proposed method was developed and validated with the aim to be used in Bioavailability/Bioequivalence studies for quantification of drug in human plasma. The mobile phase components were acetonitrile, methanol, and water in the ratio of 20:50:30. Ortho phosphoric acid was used to adjust at pH5.0. Flow rate and wavelength were kept 1ml/min and 247nm respectively. The column was C-18 HPLC column 5um particle size, L x 1.d. 25cm x4.6mm. (Supelcosil). Retention time of Cefpodoxime Proxetil was found to be 10.967min. The developed method was validated for selectivity, recovery, accuracy, precision, repeatability, reproducibility, stability and linearity in the range of 0.195mcg/ml to 50mcg/ml. The accuracy and Precision of the proposed method were well within the predefined limits i.e. ±15% for all the calibration standards other than LLOQ (Lower Limit of Quantification) where it was well within ±20% of the nominal value. The analytical recovery was always above 89% showing satisfactory recovery. The coefficient of correlation (R2 ) was 0.999. The developed method was found suitable for the estimation of Cefpodoxime Proxetil in plasma. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi en_US
dc.subject Cefpodoxime Proxetil en_US
dc.subject HPLC en_US
dc.subject validation en_US
dc.subject human plasma en_US
dc.title Development and validation of HPLC method for the determination of Cefpodoxime Proxetil in human plasma en_US
dc.type Article en_US


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