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Model design for screening effective Antihyperlipidemic drugs using zebrafish system

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dc.contributor.author Chen, Kan
dc.contributor.author Wang, Chang-qian
dc.contributor.author Fan, Yu-qi
dc.contributor.author Xie, Yu-shui
dc.contributor.author Yin, Zhao-fang
dc.contributor.author Xu, Zuo-jun
dc.contributor.author Zhang, Hui-li
dc.contributor.author Cao, Jia-tian
dc.contributor.author Wang, Yue
dc.contributor.author Gao, Lin
dc.date.accessioned 2022-11-23T05:18:09Z
dc.date.available 2022-11-23T05:18:09Z
dc.date.issued 2017-09-25
dc.identifier.citation Chen, K., Wang, C. Q., Fan, Y. Q., Xie, Y. S., Yin, Z. F., Xu, Z. J., ... & Gao, L. (2017). Model design for screening effective Antihyperlipidemic drugs using zebrafish system. Pakistan journal of pharmaceutical sciences, 30(5). en_US
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14090
dc.description.abstract The purpose of this paper was to explore a new method for screening lipid-lowering drugs in zebrafish models. The suitable drug concentrations of atorvastatin (ATV), fenofibrate (FEF) and ezetimibe (EZE) were first determined. Then, the serum cholesterol and triglyceride levels were detected in high-fat diet (HFD)-fed zebrafish. The HFD zebrafish models were constructed and the effects of drugs on them were observed by Oil red O staining and fluorescence labeling. Statistical analyses among groups were conducted using SPSS software. The lowest drug concentration (LDC) and the highest (HDC) of ATV, FEF and EZE were 0.3 µM/37.0µM, 1.2µM/3.5µM, and 6.3 µM/26.4µM, respectively, while, the intermediate (IDC) was, in order, 18.5µM, 1.8µM, 13.2µM. The cholesterol and triglyceride levels in HFD-fed zebrafish were increased after 7 weeks fat feeding (p<0.05). Moreover, the levels of triglyceride were significantly decreased after LDC of ATV and FEF treated (p<0.05), but not that of EZE. While, the cholesterol levels were reduced in three groups (p<0.05). Moreover, the 5 dpf high-fat zebrafish model was established successfully and maintained stably for 24h. ATV produced effects in a concentration-dependent manner, while only IDC and HDC of FEF and EZE made effects on this model. Intravascular cholesterol levels were significantly increased after HCD treatment and decreased after drug treated. The high-fat zebrafish model induced by HFD-fed was available and successful, besides, the Oil red O staining may be an available and rapid method for screening lipid-lowering drugs. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachi en_US
dc.subject zebrafish en_US
dc.subject hyperlipidemia en_US
dc.subject lipid-lowering drug en_US
dc.subject cholesterol en_US
dc.subject triglyceride en_US
dc.title Model design for screening effective Antihyperlipidemic drugs using zebrafish system en_US
dc.type Article en_US


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