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Screening of medicinally important Berberis lyceum for their antimicrobial activity by disc diffusion assay

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dc.contributor.author Bakht, Jehan
dc.contributor.author Iftikhar, Zainab
dc.contributor.author Shafi, Mohammad
dc.contributor.author Iqbal, Arshad
dc.date.accessioned 2022-11-28T04:46:05Z
dc.date.available 2022-11-28T04:46:05Z
dc.date.issued 2017-10-05
dc.identifier.citation Bakht, J., Iftikhar, Z., Shafi, M., & Iqbal, A. (2017). Screening of medicinally important Berberis lyceum for their antimicrobial activity by disc diffusion assay. Pakistan Journal of Pharmaceutical Sciences, 30(5). en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14102
dc.description.abstract The present research was focused on the anti-microbial activities of different solvent extracted fractions from commercial available and fresh plants of Berberis lyceum against Gram positive, Gram-negative bacteria and fungi using 1 and 2 mg disc-1 concentrations. Our results showed that fractions from both sources revealed different degree of antimicrobial activities. Our result indicated that Escherichia coli, Citorbacter freundii and Candida albicans were more susceptible to crude methanolic extract and the same microbes were resistant to water extracted fractions. Similarly, maximum reduction in the growth of Pseudomonas aeruginosa, Bacillus subtilis and Xanthomonas campestris was measured by hexane-extracted fractions and minimum growth inhibition by water-extracted fractions. Klebsiella pneumoniae and Staphylococcus aureus were more susceptible to ethyl acetate fraction. Majority of the tested microbes were resistant to water and butanol extracted samples. Staphylococcus aureus was the most susceptible gram-positive bacteria and Bacillus subtilis was resistant one. Among Gram-negative bacteria, Citorbacter freundii showed maximum susceptibility while Xanthomonas campestris revealed resistivity. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachi en_US
dc.subject Antibacterial en_US
dc.subject antifungal activity en_US
dc.subject Berberis lyceum en_US
dc.subject disc diffusion assay en_US
dc.title Screening of medicinally important Berberis lyceum for their antimicrobial activity by disc diffusion assay en_US
dc.type Article en_US


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