Abstract:
A crude phenolic glycolipid extract from Mycobacterium bovis BCG was fractionated by column
chromatography. A reversed-phase high performance liquid chromatography (HPLC) method with UV
detection at 275nm was developed for simultaneous detection and separation of phenolic glycolipids (PGLs) in
Mycobacterium bovis BCG. This analysis provides a good resolution. Different solvent systems and columns
for HPLC were compared. A system composed of acetonitrile-water in the ratio of 0→80% at a flow rate of 0.8
mL/min and C8 analytical column were found to be optimum for HPLC of the phenolic glycolipids. This simple
method is therefore appropriate to purify these compounds present in M. bovis extract.
