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SEPARATION OF PHENOLIC GLYCOLIPIDS IN MYCOBACTERIUM BOVIS BCG BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

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dc.contributor.author SALIMI, MONA
dc.contributor.author POURABDI, LADAN
dc.contributor.author ZAKERI, MARYAM
dc.contributor.author ABDIPOUR, NAZANIN
dc.date.accessioned 2022-11-28T09:36:45Z
dc.date.available 2022-11-28T09:36:45Z
dc.date.issued 2009-07-05
dc.identifier.citation Salimi, M., Pourabdi, L., Zakeri, M., & Abdipour, N. (2009). Separation of phenolic glycolipids in mycobacterium bovis BCG by reversed-phase high performance liquid chromatography. Pakistan Journal of Pharmaceutical Sciences, 22(2). en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14123
dc.description.abstract A crude phenolic glycolipid extract from Mycobacterium bovis BCG was fractionated by column chromatography. A reversed-phase high performance liquid chromatography (HPLC) method with UV detection at 275nm was developed for simultaneous detection and separation of phenolic glycolipids (PGLs) in Mycobacterium bovis BCG. This analysis provides a good resolution. Different solvent systems and columns for HPLC were compared. A system composed of acetonitrile-water in the ratio of 0→80% at a flow rate of 0.8 mL/min and C8 analytical column were found to be optimum for HPLC of the phenolic glycolipids. This simple method is therefore appropriate to purify these compounds present in M. bovis extract. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachi en_US
dc.subject HPLC-UV en_US
dc.subject mycobacterium en_US
dc.subject phenolic glycolipid en_US
dc.title SEPARATION OF PHENOLIC GLYCOLIPIDS IN MYCOBACTERIUM BOVIS BCG BY REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY en_US
dc.type Article en_US


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