Abstract:
Fifty strains of genus Vibrio were isolated (identified) from healthy and diseased marine catfish(es). The
isolates were screened for bacteriocin (vibriocin) production. About 32% isolates were found bacteriocin
producers. The best producer was identified as Vibrio anguillarum AVP10. The maximum production of
vibriocin AVP10 was manifested at 29°C at pH 7, after 18-20h of incubation. Vibriocin activity was enhanced
in the presence of citrate-phosphate buffer. The vibriocin AVP10 withstands autoclaving temperature and
showed activity even after prolonged chloroform treatment. Proteolytic enzymes inhibited its activity, while
lipolytic enzyme had no effect. It was found bioactive only against intrageneric bacterial strains. Mode of
action of vibriocin AVP10 varies with the indicator (sensitive) culture used i.e. bactericidal effects was exerted
against V. anguillarum AVS9 while bacteriostatic effect was shown against entero-toxigenic E. coli.
