Abstract:
A new, simple, precise, rapid and selective high-performance thin-layer chromatographic (HPTLC) method for
the simultaneous quantification of Metronidazole (MTZ) and Miconazole nitrate (MCZ) in gel has been
developed. It was performed on silica gel 60 GF254 Thin Layer Chromatographic plates using mobile phase
comprising of Toluene: Chloroform: Methanol (3.0:2.0:0.6 v/v) and the detection was carried out at 240 nm
using densitometer. The retention factors of MTZ and MCZ were 0.34 and 0.55 respectively. Calibration curves
were linear in the range of 300-700 ng/spot of MTZ and 600-1400 ng/spot of MCZ both by height and by area.
The percent recovery of the drugs from gel carried out by standard addition method was found to be
100.13±1.59 (by height) and 98.92±0.76 (by area) for MTZ and 99.49±1.58 (by height) and 99.63±1.46 (by
area) for MCZ indicative of accuracy and precision of simultaneous determination of MTZ and MCZ nitrate.
