Abstract:
A simple, specific and accurate reverse phase liquid chromatographic method has been developed for the
simultaneous determination of naproxen and ranitidine HCl. Both the drugs are official with British
Pharmacopoeia 2007, but do not involve simultaneous determination of naproxen and ranitidine HCl. The
separation was carried out using 4.6×250 mm Symmetry Shield TM RP 18 with a particle diameter of 5 µm and
mobile phase containing 0.1M orthophosphoric acid: methanol (35:65, pH 3.1) in isocratic mode. The flow rate
was 1.00 ml/min and effluent was monitored at 240 nm. The retention times (average) of ranitidine HCl and
naproxen were 2.36 min and 12.39 min, respectively. The linearity for naproxen and ranitidine HCl was in the
range of 5-35 µg/ ml and 1.5-12 µg/ ml, respectively. The potencies of naproxen and ranitidine HCl were found
99.40 % and 99.48 %, respectively. The proposed method was validated and successfully applied to the
estimation of naproxen and ranitidine HCl in newly formulated combined tablet and in plasma.
