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CYTOTOXICITY OF VIBRIO CHOLERAE ON MADIN DARBY BOVINE KIDNEY CELLS

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dc.contributor.author MANJURUL HAQUE, QUAZI
dc.contributor.author FADILAH MOHAMAD, NAJMIYATUL
dc.contributor.author HELAL UDDIN, ABM
dc.contributor.author SAEED, MOHAMMAD
dc.date.accessioned 2022-11-30T09:22:24Z
dc.date.available 2022-11-30T09:22:24Z
dc.date.issued 2010-10-07
dc.identifier.citation Haque, Q. M., Mohamad, N. F., Uddin, A. H., & Saeed, M. (2010). Cytotoxicity of Vibrio cholerae on Madin Darby Bovine Kidney cells. Pakistan Journal of Pharmaceutical Sciences, 23(4), 393-398. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14262
dc.description.abstract The cytotoxicity of cell-free culture filtrates of 31 isolates of Vibrio cholerae O1 and O139, 5 reference strains and 26 clinical isolates, was tested on Madin Darby Bovine Kidney (MDBK) cells and Vero cells. The 3-[4,5- dimethylthiazol-2-y]-2, 5-diphenyltetrazolium bromide (MTT) test was used to detect the effect of the filtrates on the proliferation and viability of cultured cell populations. The filtrates were prepared from serial ten-fold dilutions of inoculated AKI and APW broth media with and without the addition of polymyxin B. The APW culture filtrates of both V. cholerae O1 and O139 with and without added polymyxin B showed greater toxicity to MDBK cells as compared to AKI filtrates. The cytotoxicity of AKI-grown V. cholerae O139 to MDBK cells was greater than that of V. cholerae O1 grown in the same medium. The cytotoxicity of APW filtrates on Vero cells was low and only noted when polymyxin was added to the medium. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachi en_US
dc.subject Vibrio cholerae en_US
dc.subject MDBK cells en_US
dc.subject Vero cells en_US
dc.subject Cytotoxicity and MTT test en_US
dc.title CYTOTOXICITY OF VIBRIO CHOLERAE ON MADIN DARBY BOVINE KIDNEY CELLS en_US
dc.type Article en_US


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