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DEVELOPMENT AND VALIDATION OF PCR, PLS, AND TLC DENSITOMETRIC METHODS FOR THE SIMULTANEOUS DETERMINATION OF VITAMINS B1, B6 AND B12 IN PHARMACEUTICAL FORMULATIONS

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dc.contributor.author S. ELZANFALY, EMAN
dc.contributor.author NEBSEN, MARIANNE
dc.contributor.author K. RAMADAN, NESREEN
dc.date.accessioned 2022-11-30T09:32:44Z
dc.date.available 2022-11-30T09:32:44Z
dc.date.issued 2010-10-10
dc.identifier.citation Elzanfaly, E. S., Nebsen, M., & Ramadan, N. K. (2010). Development and validation of PCR, PLS and TLC densitometric methods for the simultaneous determination of vitamins B1, B6 and B12 in pharmaceutical formulations. Pak. J. Pharm. Sci, 23(4), 409-415. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/14265
dc.description.abstract This work represents the simultaneous determination of thiamine hydrochloride (B1), pyridoxine hydrochloride (B6) and cyanocobalamine (B12) by two different methods namely spectrophotometry multivariate calibration and densitometry. The spectrophotometric numerical method depends on the use of spectrophotometric data coupled to PLS and PCR multivariate calibration methods for the simultaneous determination of (B1) and (B6) in the presence of (B12) in laboratory prepared mixtures and commercial tablets. A calibration set was prepared, where the three vitamins were modeled using a full factorial 23 with three center points experimental design. This calibration set was used to build the PLS and PCR models. The models were validated by testing their predictive ability on a validation set where low RMSEP, RSEP % were obtained for both models. Figures of merit were determined using the net analyte signal concept. The proposed models were applied successfully to simultaneous determination of B1 and B6 in presence of a low concentration of B12 in pharmaceutical dosage forms that contain simple excipients. The TLC densitometric method was based on the use of a developing system of chloroform: ethanol: water: acetic acid solution (2: 8: 2: 0.5 v/v) to separate the three vitamins. The separated spots were scanned at 242nm, 291nm and 360nm for B1, B6 and B12 respectively. The proposed method was applied successfully to simultaneous determination of the three vitamins in their pure powder form in the range 0.1-1.5 (µg/spot), 0.5-3.5 (µg/spot), 0.1-1.5 (µg/spot) for B1, B6, and B12 respectively and in their pharmaceutical formulations. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences, Karachi en_US
dc.subject Thiamine hydrochloride (B1) en_US
dc.subject Pyridoxine hydrochloride (B6) en_US
dc.subject Cyanocobalamin (B12) en_US
dc.subject PLS en_US
dc.subject PCR en_US
dc.subject densitometry en_US
dc.title DEVELOPMENT AND VALIDATION OF PCR, PLS, AND TLC DENSITOMETRIC METHODS FOR THE SIMULTANEOUS DETERMINATION OF VITAMINS B1, B6 AND B12 IN PHARMACEUTICAL FORMULATIONS en_US
dc.type Article en_US


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