Abstract:
Tamarindus indica Linn was used as a traditional medicine for the management of diabetes mellitus in human
and experimental animals. This study investigated effects of aqueous extract of Tamarindus indica seeds
(AETIS) against STZ-induced damages in pancreatic islands by means of stereological methods. sixty matured
normoglycemic male Wistar rats, weighing 200- 250 gr, were selected and randomly divided into 6 groups
(n=10). Control, STZ-induced diabetic; by intraperitoneal injection of 55 mg/Kg streptozotocin, Treated control
group (TC); received AETIS at a dose of 200mg/kg/day, and AETIS treated diabetic groups (TD1-3); received
respectively AETIS at the dose of 50, 100,and 200 mg/kg/day by gavage from one week after induction of
diabetes by STZ. After 8 weeks of experiment, stereological estimation of volume density and total volume of
islets and beta cells, volume weighted mean islets volume, mass of beta cells, islets, and pancreas and total
number of islets were done. Volume density and total volume of islets, volume weighted mean islets volume,
volume density islets/pancreas, volume density beta cells/islet, mass of islets and pancreas of treated diabetic
groups (TD1-3) were significantly higher than untreated diabetic group (P<0.001), and in TD3 group these values
were comparable to controls. Although total volume and mass of beta cells in TD1-3 were significantly higher
than D group but they were significantly lower than control group (P<0.05). Total number of islets, pancreas
wet weight and volume did not show any significant changes between control and experimental groups
(P>0.05). Results suggested that AETIS partially restores pancreatic beta cells and repairs STZ- induced
damages in rats.
