OPTIMIZATION OF THE CULTURE CONDITIONS IN THE RICE (ORYZA SATIVA CV. BASMATI-370)

Abstract

The objective of the present study was to develop an efficient callus induction protocol for a fine rice (Oryza sativa L.) variety Basmati-370. Mature grains of this variety were used for callus induction. The Chu’s N6 medium was used as basal medium for callus induction and was supplemented with sucrose, Myo-inositol, Casein, agar (4 g L-1, 5 g L-1, 6 g L-1) and growth hormone 2,4-Dichlorophenoxy acetic acid in different concentrations of 2ppm and 2.5ppm. The genotype responded well for all the three concentrations of agar (4 g L-1, 5 g L-1, 6 g L-1) and two concentrations of 2,4-D (2ppm, 2.5ppm), however, the combination of 4 g L-1 agar with 2.5ppm 2,4-D gave excellent results with maximum callus induction of 93.42% followed by 4 g L-1 agar with 2ppm 2,4-D and 5 g L-1 with 2.5ppm 2,4-D which also gave better results with the observed callus induction of 92.75% and 91.11%, respectively.