PRODUCTION POTENTIAL OF LOCALLY ISOLATED STRAIN OF FUSARIUM SOLANI (MBL 24) FOR EXTRACELLULAR LIPASES

Abstract

The present study is focused to exploit the indigenous strain of Fusarium solani (MBL-24) for the extracellular lipases production through solid state fermentation technique. In order to exploit the locally isolated fungal strain for the industrial usage, various parameters tested was incubation temperature, screening of substrates, incubation period, size of inoculum, volume of moistening agent, additional oils, additional carbon and nitrogen sources. Wheat bran was surprisingly found to be an ideal source for the growth of the organism and subsequently for enhanced enzyme production as compared to the other substrates. Out of various oils tested brassica oil at 1% was found to be an ideal additional source for the maximum activity (3.78±0.04a U/mL) of lipases. It was found that 2 mL of inoculum supported highest lipase enzyme (4.32±0.05a U/mL) production. Maximum activity (5.36±0.10a U/mL) was obtained when fungus was incubated for two days (48h) and at 1:3 substrate to diluent ratio i.e., (7.0 ± 0.03 a U/mL). As an additional supplement 1% CaCO3 was proved to be best for maximum extracellular lipases production. Inorganic nitrogen source seems to be better supplement as compared to organic nitrogen sources. Sodium nitrate supported maximum lipase production (5.17 ± 0.07 a U/mL). It is apparent from the optimized conditions that the strain has a good potential for the production of extracellular lipases and can be exploited for several industries keeping in view the optimized temperature and incubation period.