BIOLOGICAL SCREENING OF OILS FROM IMPATIENS BICOLOR ROYLE

Abstract

The oil sub-fractions obtained by solid phase extraction of the n-hexane fraction from aerial parts of the Impatiens bicolor Royle, were tested In vitro for their antibacterial, antifungal, insecticidal, cytotoxic and phytotoxic activities. Bacterial strains used for the experiment were Shigella flexeneri, Escherchia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi and Staphylococcus aureus while fungal strains included Candida albicans, Aspergillus flavus, Fusarium solani, Microspoum canis, Candida glaberatan and Trichophyton longifusis. The tested samples did not display any activity against above mentioned bacterial strains while in case of antifungal activity, fractions MQ-6 and MQ-7 exhibited low activity against Microsporum canis. The crude oil sub-fraction MQ-4 (81.25%) exhibited highly significant phytotoxicity in the tested concentration (1000 mg/mL) against Lemna plant. The tested samples revealed low to moderated insecticidal activity against Tribolium castaneum while in case of brine shrimp lethality, MQ-5, MQ-6, MQ-7 showed significant cytotoxicity at high dose 1000 µg/mL with LD50 value 170.748, 170.79, 161.54 µg/ mL respectively.